CATH Domain 5ks2A00

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.40	3-Layer(aba) Sandwich
	3.40.50	Rossmann fold
	3.40.50.150	Vaccinia Virus protein VP39

Domain Context

CATH Clusters

Superfamily	Vaccinia Virus protein VP39
Functional Family

Enzyme Information

2.1.1.226

23S rRNA (cytidine(1920)-2'-O)-methyltransferase.

based on mapping to UniProt P9WJ63

S-adenosyl-L-methionine + cytidine(1920) in 23S rRNA = S-adenosyl-L- homocysteine + 2'-O-methylcytidine(1920) in 23S rRNA.

-!- The bifunctional enzyme from Mycobacterium tuberculosis 2'-O- methylates cytidine(1920) in helix 69 of 23S rRNA and cytidine(1409) in helix 44 of 16S rRNA (cf. EC 2.1.1.227). -!- These methylations result in increased susceptibility to the antibiotics capreomycin and viomycin.

2.1.1.227

16S rRNA (cytidine(1409)-2'-O)-methyltransferase.

based on mapping to UniProt P9WJ63

S-adenosyl-L-methionine + cytidine(1409) in 16S rRNA = S-adenosyl-L- homocysteine + 2'-O-methylcytidine(1409) in 16S rRNA.

-!- The bifunctional enzyme from Mycobacterium tuberculosis 2'-O- methylates cytidine(1409) in helix 44 of 16S rRNA and cytidine(1920) in helix 69 of 23S rRNA (cf. EC 2.1.1.226).

UniProtKB Entries (1)

P9WJ63

TLYA_MYCTU

Mycobacterium tuberculosis H37Rv

16S/23S rRNA (cytidine-2'-O)-methyltransferase TlyA

PDB Structure

PDB	5KS2
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	A Novel Motif for S-Adenosyl-l-methionine Binding by the Ribosomal RNA Methyltransferase TlyA from Mycobacterium tuberculosis. Witek, M.A., Kuiper, E.G., Minten, E., Crispell, E.K., Conn, G.L. J. Biol. Chem.