CATH Domain 2h8cA00

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.30	2-Layer Sandwich
	3.30.1330	60s Ribosomal Protein L30; Chain: A;
	3.30.1330.70	Holliday junction resolvase RusA

Domain Context

CATH Clusters

Superfamily	Holliday junction resolvase RusA
Functional Family	Crossover junction endodeoxyribonuclease RusA

Enzyme Information

3.1.22.4

Crossover junction endodeoxyribonuclease.

based on mapping to UniProt P0AG74

Endonucleolytic cleavage at a junction such as a reciprocal single- stranded crossover between two homologous DNA duplexes (Holliday junction).

-!- The enzyme from Saccharomyces cerevisiae has no endonuclease or exonuclease activity on single-stranded or double-stranded DNA molecules that do not contain Holliday junctions.

UniProtKB Entries (1)

P0AG74

RUSA_ECOLI

Escherichia coli K-12

Crossover junction endodeoxyribonuclease RusA

PDB Structure

PDB	2H8C
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism	Escherichia
Primary Citation	RusA Holliday junction resolvase: DNA complex structure--insights into selectivity and specificity. Macmaster, R., Sedelnikova, S., Baker, P.J., Bolt, E.L., Lloyd, R.G., Rafferty, J.B. Nucleic Acids Res.