The distribution of vacuoles into daughter cells after mitosis or meiosis, mediated by interactions between vacuoles and the cytoskeleton.

The process of directing proteins towards the vacuole, usually using signals contained within the protein.

The directed movement of proteins in a cell, including the movement of proteins between specific compartments or structures within a cell, such as organelles of a eukaryotic cell.

The directed movement of substances from the Golgi to early sorting endosomes. Clathrin vesicles transport substances from the trans-Golgi to endosomes.

The directed movement of substances from the Golgi to the vacuole.

A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of a vacuole.

Any process that reduces the pH of the vacuole, measured by the concentration of the hydrogen ion.

A cytoplasm to vacuole targeting pathway that uses machinery common with autophagy. The Cvt vesicle is formed when the receptor protein, Atg19, binds to the complexes of the target protein (aminopeptidase or alpha-mannosidase homododecamers), forming the Cvt complex. Atg11 binds to Atg9 and transports the Cvt complex to the pre-autophagosome (PAS). The phagophore membrane expands around the Cvt complex (excluding bulk cytoplasm) forming the Cvt vesicle. This pathway is mostly observed in yeast.

Any process that increases the frequency, rate or extent of assembly of the SNARE complex. The SNARE complex is a protein complex involved in membrane fusion; a stable ternary complex consisting of a four-helix bundle, usually formed from one R-SNARE and three Q-SNAREs with an ionic layer sandwiched between hydrophobic layers.

The joining of the lipid bilayer membrane around a Golgi transport vesicle to the target lipid bilayer membrane.

The directed movement of substances from the Golgi to the vacuole.

The joining of the lipid bilayer membrane around a Golgi transport vesicle to the target lipid bilayer membrane.

The lipid bilayer surrounding any of the compartments of the Golgi apparatus.

The network of interconnected tubular and cisternal structures located within the Golgi apparatus on the side distal to the endoplasmic reticulum, from which secretory vesicles emerge. The trans-Golgi network is important in the later stages of protein secretion where it is thought to play a key role in the sorting and targeting of secreted proteins to the correct destination.

The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.

The lipid bilayer surrounding a vacuole that retains the same shape regardless of cell cycle phase. The membrane separates its contents from the cytoplasm of the cell. An example of this component is found in Arabidopsis thaliana.

The component of a membrane consisting of gene products and protein complexes that are loosely bound to one of its surfaces, but not integrated into the hydrophobic region.

A protein complex involved in membrane fusion; a stable ternary complex consisting of a four-helix bundle, usually formed from one R-SNARE and three Q-SNAREs with an ionic layer sandwiched between hydrophobic layers. One well-characterized example is the neuronal SNARE complex formed of synaptobrevin 2, syntaxin 1a, and SNAP-25.

The lipid bilayer surrounding any of the compartments of the Golgi apparatus.

A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

A vacuole to which materials ingested by endocytosis are delivered.

The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.

A membrane-bounded intracellular vesicle that arises from the ingestion of particulate material by phagocytosis.