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CATH Superfamily 3.40.50.150

Vaccinia Virus protein VP39

The name of this superfamily has been modified since the most recent official CATH+ release (v4_3_0). At the point of the last release, this superfamily was named:

"
Vaccinia Virus protein VP39
".

Functional Families

Overview of the Structural Clusters (SC) and Functional Families within this CATH Superfamily. Clusters with a representative structure are represented by a filled circle.
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FunFam 112: tRNA (Cytosine-5-)-methyltransferase NCL1

There are 2 EC terms in this cluster

Please note: EC annotations are assigned to the full protein sequence rather than individual protein domains. Since a given protein can contain multiple domains, it is possible that some of the annotations below come from additional domains that occur in the same protein, but have been classified elsewhere in CATH.

Note: The search results have been sorted with the annotations that are found most frequently at the top of the list. The results can be filtered by typing text into the search box at the top of the table.

EC Term Annotations Evidence
Multisite-specific tRNA:(cytosine-C(5))-methyltransferase. [EC: 2.1.1.202]
(1) S-adenosyl-L-methionine + cytosine(34) in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine(34) in tRNA precursor. (2) S-adenosyl-L-methionine + cytosine(40) in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine(40) in tRNA precursor. (3) S-adenosyl-L-methionine + cytosine(48) in tRNA = S-adenosyl-L- homocysteine + 5-methylcytosine(48) in tRNA. (4) S-adenosyl-L-methionine + cytosine(49) in tRNA = S-adenosyl-L- homocysteine + 5-methylcytosine(49) in tRNA.
  • The enzyme from Saccharomyces cerevisiae is responsible for complete 5-methylcytosine methylations of yeast tRNA.
  • The incidence of modification depends on the cytosine position in tRNA.
  • At positions 34 and 40, 5-methylcytosine is found only in two yeast tRNAs (tRNA(Leu)(CUA) and tRNA(Phe)(GAA), respectively), whereas most other elongator yeast tRNAs bear either 5-methylcytosine(48) or 5-methylcytosine(49), but never both in the same tRNA molecule.
  • The formation of 5-methylcytosine(34) and 5-methylcytosine(40) is a strictly intron-dependent process, whereas the formation of 5-methylcytosine(48) and 5-methylcytosine(49) is an intron- independent process.
  • Formerly EC 2.1.1.29.
 9 G2W8Y5 G2W8Y5 G2W8Y5 N1P7E7 N1P7E7 N1P7E7 P38205 P38205 P38205
TRNA (cytosine(34)-C(5))-methyltransferase. [EC: 2.1.1.203]
S-adenosyl-L-methionine + cytosine(34) in tRNA precursor = S-adenosyl-L- homocysteine + 5-methylcytosine(34) in tRNA precursor.
  • The human enzyme is specific for C(5)-methylation of cytosine(34) in tRNA precursors.
  • The intron in the human pre-tRNA(Leu)(CAA) is indispensable for the C(5)-methylation of cytosine in the first position of the anticodon.
  • It is not able to form 5-methylcytosine at positions 48 and 49 of human and yeast tRNA precursors.
  • Formerly EC 2.1.1.29.
 1 Q9W4M9
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