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CATH Superfamily 1.10.630.10

Cytochrome P450

The name of this superfamily has been modified since the most recent official CATH+ release (v4_3_0). At the point of the last release, this superfamily was named:

"
Cytochrome P450
".

Functional Families

Overview of the Structural Clusters (SC) and Functional Families within this CATH Superfamily. Clusters with a representative structure are represented by a filled circle.
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FunFam 11: Cytochrome P450 83B1

There are 5 EC terms in this cluster

Please note: EC annotations are assigned to the full protein sequence rather than individual protein domains. Since a given protein can contain multiple domains, it is possible that some of the annotations below come from additional domains that occur in the same protein, but have been classified elsewhere in CATH.

Note: The search results have been sorted with the annotations that are found most frequently at the top of the list. The results can be filtered by typing text into the search box at the top of the table.

EC Term Annotations Evidence
(Methylsulfanyl)alkanaldoxime N-monooxygenase. [EC: 1.14.14.43]
An (E)-omega-(methylthio)alkanal oxime + O(2) + glutathione + [reduced NADPH--hemoprotein reductase] = an (E)-1-(glutathione-S-yl)-omega- (methylthio)-alkylhydroximate + 2 H(2)O + [oxidized NADPH--hemoprotein reductase].
  • Involved in the biosynthesis of glucosinolates in plants.
  • Catalyzes an N-hydroxylation of the E isomer of n-(methylsulfanyl)aldoximes, forming an aci-nitro intermediate that reacts non-enzymically with glutathione to produce an N-alkyl- thiohydroximate adduct, the committed precursor of glucosinolates.
  • In the absence of a thiol compound, the enzyme is suicidal, probably due to interaction of the reactive aci-nitro intermediate with active site residues.
 4 A0A178UZS6 A0A178UZS6 P48421 P48421
Aromatic aldoxime N-monooxygenase. [EC: 1.14.14.45]
(1) (E)-indol-3-ylacetaldehyde oxime + [reduced NADPH--hemoprotein reductase] + glutathione + O(2) = S-((E)-N-hydroxy(indol-3- yl)acetimidoyl)-L-glutathione + [oxidized NADPH--hemoprotein reductase] + 2 H(2)O. (2) (E)-phenylacetaldehyde oxime + [reduced NADPH--hemoprotein reductase] + glutathione + O(2) = S-((Z)-N-hydroxy(phenyl)acetimidoyl)-L-glutathione + [oxidized NADPH--hemoprotein reductase] + 2 H(2)O (overall reaction).
  • Involved in the biosynthesis of glucosinolates in plants.
  • The enzyme catalyzes the N-hydroxylation of aromatic aldoximes derived from L-tryptophan and L-phenylalanine, forming an aci-nitro intermediate that reacts non-enzymically with glutathione to produce an N-alkyl-thiohydroximate adduct, the committed precursor of glucosinolates.
  • In the absence of glutathione, the enzyme is suicidal, probably due to interaction of the reactive aci-nitro compound with catalytic residues in the active site.
 4 A0A178USK3 A0A178USK3 O65782 O65782
Psoralen synthase. [EC: 1.14.14.141]
(+)-marmesin + [reduced NADPH--hemoprotein reductase] + O(2) = psoralen + [oxidized NADPH--hemoprotein reductase] + acetone + 2 H(2)O.
  • This microsomal cytochrome P450 (heme-thiolate) enzyme is rather specific for (+)-marmesin, although it can also accept 5-hydroxymarmesin to a much lesser extent.
  • Furanocoumarins protect plants from fungal invasion and herbivore attack.
  • (+)-columbianetin, the angular furanocoumarin analog of the linear furanocoumarin (+)-marmesin, acts as a competitive inhibitor even though it is not a substrate.
  • Formerly EC 1.14.13.102.
 1 Q6QNI4
Camalexin synthase. [EC: 1.14.19.52]
2-(L-cystein-S-yl)-2-(1H-indol-3-yl)acetonitrile + 2 [reduced NADPH- -hemoprotein reductase] + 2 O(2) = camalexin + hydrogen cyanide + CO(2) + 2 [oxidized NADPH--hemoprotein reductase] + 4 H(2)O.
  • This P450 enzyme, which has been characterized from the plant Arabidopsis thaliana, catalyzes the last two steps in the biosynthesis of camalexin, the main phytoalexin in that plant.
  • The enzyme catalyzes two successive oxidation events.
  • During the first of oxidation the enzyme introduces a C-N double bond, liberating hydrogen cyanide, and during the second oxidation it catalyzes a decarboxylation.
  • Formerly EC 1.3.1.n2.
 1 Q9LW27
(+)-menthofuran synthase. [EC: 1.14.14.143]
(+)-pulegone + [reduced NADPH--hemoprotein reductase] + O(2) = (+)- menthofuran + [oxidized NADPH--hemoprotein reductase] + H(2)O.
  • A cytochrome P450 (heme-thiolate) protein.
  • The conversion of substrate into product involves the hydroxylation of the syn-methyl (C(9)), intramolecular cyclization to the hemiketal and dehydration to the furan.
  • This is the second cytochrome P450-mediated step of monoterpene metabolism in peppermint, with the other step being catalyzed by EC 1.14.14.99.
  • Formerly EC 1.14.13.104.
 1 Q947B7
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