CATH Classification
Level | CATH Code | Description |
---|---|---|
3 | Alpha Beta | |
3.10 | Roll | |
3.10.129 | Thiol Ester Dehydrase; Chain A | |
3.10.129.10 | Hotdog Thioesterase |
Domain Context
CATH Clusters
Superfamily | Hotdog Thioesterase |
Functional Family | 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ |
Enzyme Information
4.2.1.59 |
3-hydroxyacyl-[acyl-carrier-protein] dehydratase.
based on mapping to UniProt P0A6Q6
A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl- carrier protein] + H(2)O.
-!- This enzyme is responsible for the dehydration step of the dissociated (type II) fatty-acid biosynthesis system that occurs in plants and bacteria. -!- The enzyme uses fatty acyl thioesters of ACP in vivo. -!- Different forms of the enzyme may have preferences for substrates with different chain length. -!- For example, the activity of FabZ, the ubiquitous enzyme in bacteria, decreases with increasing chain length. -!- Gram-negative bacteria that produce unsaturated fatty acids, such as Escherichia coli, have another form (FabA) that prefers intermediate chain length, and also catalyzes EC 5.3.3.14. -!- Despite the differences both forms can catalyze all steps leading to the synthesis of palmitate (C16:0). -!- FabZ, but not FabA, can also accept unsaturated substrates. -!- Formerly EC 4.2.1.58, EC 4.2.1.60 and EC 4.2.1.61.
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UniProtKB Entries (1)
P0A6Q6 |
FABZ_ECOLI
Escherichia coli K-12
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ
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PDB Structure
PDB | 6N3P |
External Links | |
Method | X-RAY DIFFRACTION |
Organism | |
Primary Citation |
Structural and dynamical rationale for fatty acid unsaturation inEscherichia coli.
Proc. Natl. Acad. Sci. U.S.A.
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