CATH Classification

Domain Context

CATH Clusters

Superfamily Inhibitor Of Apoptosis Protein (2mihbC-IAP-1); Chain A
Functional Family E3 ubiquitin-protein ligase XIAP

Enzyme Information

2.3.2.27
RING-type E3 ubiquitin transferase.
based on mapping to UniProt P98170
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine.
-!- RING E3 ubiquitin transferases serve as mediators bringing the ubiquitin-charged E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) and an acceptor protein together to enable the direct transfer of ubiquitin through the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon-amino group of an L-lysine residue of the acceptor protein. -!- Unlike EC 2.3.2.26 the RING-E3 domain does not form a catalytic thioester intermediate with ubiquitin. -!- Many members of the RING-type E3 ubiquitin transferase family are not able to bind a substrate directly, and form a complex with a cullin scaffold protein and a substrate recognition module (the complexes are named CRL for Cullin-RING-Ligase). -!- In these complexes, the RING-type E3 ubiquitin transferase provides an additional function, mediating the transfer of a NEDD8 protein from a dedicated E2 carrier to the cullin protein (see EC 2.3.2.32). -!- Cf. EC 2.3.2.31.

UniProtKB Entries (1)

P98170
XIAP_HUMAN
Homo sapiens
E3 ubiquitin-protein ligase XIAP

PDB Structure

PDB 5OQW
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
ASTX660, a Novel Non-peptidomimetic Antagonist of cIAP1/2 and XIAP, Potently Induces TNF alpha-Dependent Apoptosis in Cancer Cell Lines and Inhibits Tumor Growth.
Ward, G.A., Lewis, E.J., Ahn, J.S., Johnson, C.N., Lyons, J.F., Martins, V., Munck, J.M., Rich, S.J., Smyth, T., Thompson, N.T., Williams, P.A., Wilsher, N.E., Wallis, N.G., Chessari, G.
Mol. Cancer Ther.