CATH Classification

Domain Context

CATH Clusters

Superfamily Rhomboid-like
Functional Family Rhomboid protease GlpG

Enzyme Information

3.4.21.105
Rhomboid protease.
based on mapping to UniProt P09391
Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.
-!- These endopeptidases are multi-spanning membrane proteins. -!- Their catalytic site is embedded within the membrane and they cleave type-1 transmembrane domains. -!- Important for embryo development in Drosophila melanogaster. -!- Rhomboid is a key regulator of EGF receptor signaling and is responsible for cleaving Spitz, the main ligand of the Drosophila EGF receptor pathway. -!- Parasite-encoded rhomboid enzymes are also important for invasion of host cells by Toxoplasma and the malaria parasite. -!- Belongs to peptidase family S54.

UniProtKB Entries (1)

P09391
GLPG_ECOLI
Escherichia coli K-12
Rhomboid protease GlpG

PDB Structure

PDB 5MT8
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
General and Modular Strategy for Designing Potent, Selective, and Pharmacologically Compliant Inhibitors of Rhomboid Proteases.
Ticha, A., Stanchev, S., Vinothkumar, K.R., Mikles, D.C., Pachl, P., Began, J., Skerle, J., Svehlova, K., Nguyen, M.T.N., Verhelst, S.H.L., Johnson, D.C., Bachovchin, D.A., Lepsik, M., Majer, P., Strisovsky, K.
Cell Chem Biol