CATH Domain 5jzbA00

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.40	3-Layer(aba) Sandwich
	3.40.50	Rossmann fold
	3.40.50.1820	alpha/beta hydrolase

Domain Context

CATH Clusters

Superfamily	alpha/beta hydrolase
Functional Family	4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase

Enzyme Information

3.7.1.17

4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase.

based on mapping to UniProt P9WNH5

(1E,2Z)-3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4- oate + H(2)O = 3-((3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4- yl)propanoate + (2Z,4Z)-2-hydroxyhexa-2,4-dienoate.

-!- The enzyme is involved in the bacterial degradation of the steroid ring structure, and is involved in degradation of multiple steroids, such as testosterone, cholesterol, and sitosterol.

3.7.1.8

2,6-dioxo-6-phenylhexa-3-enoate hydrolase.

based on mapping to UniProt P9WNH5

2,6-dioxo-6-phenylhexa-3-enoate + H(2)O = benzoate + 2-oxopent-4-enoate.

-!- Cleaves the products from biphenol, 3-isopropylcatechol and 3-methylcatechol produced by EC 1.13.11.39 by ring-fission at a -CO-C bond. -!- Involved in the breakdown of biphenyl-related compounds by Pseudomonas sp.

UniProtKB Entries (1)

P9WNH5

HSAD_MYCTU

Mycobacterium tuberculosis H37Rv

4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase

PDB Structure

PDB	5JZB
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Investigation of the mycobacterial enzyme HsaD as a potential novel target for anti-tubercular agents using a fragment-based drug design approach. Ryan, A., Polycarpou, E., Lack, N.A., Evangelopoulos, D., Sieg, C., Halman, A., Bhakta, S., Eleftheriadou, O., McHugh, T.D., Keany, S., Lowe, E.D., Ballet, R., Abuhammad, A., Jacobs, W.R., Ciulli, A., Sim, E. Br. J. Pharmacol.