CATH Domain 5gmcA01

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.40	3-Layer(aba) Sandwich
	3.40.1280	Alpha/beta knot
	3.40.1280.10	SPOUT methyltransferase, trefoil knot domain

Domain Context

CATH Clusters

Superfamily	3.40.1280.10
Functional Family	Uncharacterized tRNA/rRNA methyltransferase HI_0380

Enzyme Information

2.1.1.-

Methyltransferases.

based on mapping to UniProt A0A072ZPM2

2.1.1.200

tRNA (cytidine(32)/uridine(32)-2'-O)-methyltransferase.

based on mapping to UniProt A0A072ZPM2

(1) S-adenosyl-L-methionine + cytidine(32) in tRNA = S-adenosyl-L- homocysteine + 2'-O-methylcytidine(32) in tRNA. (2) S-adenosyl-L-methionine + uridine(32) in tRNA = S-adenosyl-L- homocysteine + 2'-O-methyluridine(32) in tRNA.

-!- In Escherichia coli TrmJ is the only methyltransferase responsible for the formation of 2'-O-methylcytidine(32) in tRNA. -!- No methylation of cytosine(34) in tRNA(Leu)(CAA). -!- In vitro the enzyme 2'-O-methylates cytidine(34) of tRNA(Ser1) and uridine(32) of tRNA(Gln2).

UniProtKB Entries (1)

A0A072ZPM2

A0A072ZPM2_PSEAI

Pseudomonas aeruginosa

TRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ

PDB Structure

PDB	5GMC
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Methylation at position 32 of tRNA catalyzed by TrmJ alters oxidative stress response in Pseudomonas aeruginosa. Jaroensuk, J., Atichartpongkul, S., Chionh, Y.H., Wong, Y.H., Liew, C.W., McBee, M.E., Thongdee, N., Prestwich, E.G., DeMott, M.S., Mongkolsuk, S., Dedon, P.C., Lescar, J., Fuangthong, M. Nucleic Acids Res.