CATH Classification

Domain Context

CATH Clusters

Superfamily Uracil-DNA glycosylase-like domain
Functional Family Uracil-DNA glycosylase

Enzyme Information

3.2.2.27
Uracil-DNA glycosylase.
based on mapping to UniProt P12295
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.
-!- Uracil-DNA glycosylases are widespread enzymes that are found in all living organisms. -!- Uracil-DNA glycosylase (EC 3.2.2.27) and double-stranded uracil-DNA glycosylase (EC 3.2.2.28) form a central part of the DNA-repair machinery since they initiate the DNA base-excision repair pathway by hydrolyzing the N-glycosidic bond between uracil and the deoxyribose sugar thereby catalyzing the removal of mis-incorporated uracil from DNA.

UniProtKB Entries (1)

P12295
UNG_ECOLI
Escherichia coli K-12
Uracil-DNA glycosylase

PDB Structure

PDB 5EUG
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Crystal structure of Escherichia coli uracil DNA glycosylase and its complexes with uracil and glycerol: structure and glycosylase mechanism revisited.
Xiao, G., Tordova, M., Jagadeesh, J., Drohat, A.C., Stivers, J.T., Gilliland, G.L.
Proteins