CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.60 | 4-Layer Sandwich |
|
3.60.15 | Metallo-beta-lactamase; Chain A |
|
3.60.15.10 | Ribonuclease Z/Hydroxyacylglutathione hydrolase-like |
Domain Context
CATH Clusters
| Superfamily | Ribonuclease Z/Hydroxyacylglutathione hydrolase-like |
| Functional Family | N-acyl homoserine lactonase AiiA |
Enzyme Information
| 3.1.1.81 |
Quorum-quenching N-acyl-homoserine lactonase.
based on mapping to UniProt A3FJ64
An N-acyl-L-homoserine lactone + H(2)O = an N-acyl-L-homoserine.
-!- Acyl-homoserine lactones (AHLs) are produced by a number of bacterial species and are used by them to regulate the expression of virulence genes in a process known as quorum-sensing. -!- Each bacterial cell has a basal level of AHL and, once the population density reaches a critical level, it triggers AHL-signaling which, in turn, initiates the expression of particular virulence genes. -!- Plants or animals capable of degrading AHLs would have a therapeutic advantage in avoiding bacterial infection as they could prevent AHL- signaling and the expression of virulence genes in quorum-sensing bacteria. -!- N-(3-oxohexanoyl)-L-homoserine lactone, N-(3-oxododecanoyl)-L- homoserine lactone, N-butanoyl-L-homoserine lactone and N-(3-oxooctanoyl)-L-homoserine lactone can act as substrates.
|
UniProtKB Entries (1)
| A3FJ64 |
AHLL_BACTU
Bacillus thuringiensis
N-acyl homoserine lactonase
|
PDB Structure
| PDB | 5EHT |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Conformational Tinkering Drives Evolution of a Promiscuous Activity through Indirect Mutational Effects.
Biochemistry
|