CATH Domain 5b4fA00

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.40	3-Layer(aba) Sandwich
	3.40.50	Rossmann fold
	3.40.50.620	HUPs

Domain Context

CATH Clusters

Superfamily	HUPs
Functional Family

Enzyme Information

2.8.1.15

tRNA-5-methyluridine(54) 2-sulfurtransferase.

based on mapping to UniProt Q72LF3

ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH(2)-C(O)SH + 5-methyluracil(54) in tRNA + H(2)O = AMP + diphosphate + 5-methyl-2- thiouracil(54) in tRNA + [TtuB sulfur-carrier protein]-Gly-Gly.

-!- The enzyme, found in thermophilic bacteria such as Thermus thermophilus and archaea such as Pyrococcus horikoshii, modifies the ribothymidine (5-methyluridine) residue at position 54 of tRNAs.

UniProtKB Entries (1)

Q72LF3

TTUA_THET2

Thermus thermophilus HB27

TRNA-5-methyluridine(54) 2-sulfurtransferase

PDB Structure

PDB	5B4F
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Biochemical and structural characterization of oxygen-sensitive 2-thiouridine synthesis catalyzed by an iron-sulfur protein TtuA Chen, M., Asai, S.I., Narai, S., Nambu, S., Omura, N., Sakaguchi, Y., Suzuki, T., Ikeda-Saito, M., Watanabe, K., Yao, M., Shigi, N., Tanaka, Y. Proc. Natl. Acad. Sci. U.S.A.