CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
1 | Mainly Alpha |
|
1.10 | Orthogonal Bundle |
|
1.10.8 | Helicase, Ruva Protein; domain 3 |
|
1.10.8.590 |
Domain Context
CATH Clusters
| Superfamily | 1.10.8.590 |
| Functional Family | tRNA:Cm32/Um32 methyltransferase |
Enzyme Information
| 2.1.1.200 |
tRNA (cytidine(32)/uridine(32)-2'-O)-methyltransferase.
based on mapping to UniProt P0AE01
(1) S-adenosyl-L-methionine + cytidine(32) in tRNA = S-adenosyl-L- homocysteine + 2'-O-methylcytidine(32) in tRNA. (2) S-adenosyl-L-methionine + uridine(32) in tRNA = S-adenosyl-L- homocysteine + 2'-O-methyluridine(32) in tRNA.
-!- In Escherichia coli TrmJ is the only methyltransferase responsible for the formation of 2'-O-methylcytidine(32) in tRNA. -!- No methylation of cytosine(34) in tRNA(Leu)(CAA). -!- In vitro the enzyme 2'-O-methylates cytidine(34) of tRNA(Ser1) and uridine(32) of tRNA(Gln2).
|
UniProtKB Entries (1)
| P0AE01 |
TRMJ_ECOLI
Escherichia coli K-12
TRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ
|
PDB Structure
| PDB | 4XBO |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
tRNA recognition by a bacterial tRNA Xm32 modification enzyme from the SPOUT methyltransferase superfamily
Nucleic Acids Res.
|
