CATH Domain 4xboA01

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.40	3-Layer(aba) Sandwich
	3.40.1280	Alpha/beta knot
	3.40.1280.10	SPOUT methyltransferase, trefoil knot domain

Domain Context

CATH Clusters

Superfamily	3.40.1280.10
Functional Family	Uncharacterized tRNA/rRNA methyltransferase HI_0380

Enzyme Information

2.1.1.200

tRNA (cytidine(32)/uridine(32)-2'-O)-methyltransferase.

based on mapping to UniProt P0AE01

(1) S-adenosyl-L-methionine + cytidine(32) in tRNA = S-adenosyl-L- homocysteine + 2'-O-methylcytidine(32) in tRNA. (2) S-adenosyl-L-methionine + uridine(32) in tRNA = S-adenosyl-L- homocysteine + 2'-O-methyluridine(32) in tRNA.

-!- In Escherichia coli TrmJ is the only methyltransferase responsible for the formation of 2'-O-methylcytidine(32) in tRNA. -!- No methylation of cytosine(34) in tRNA(Leu)(CAA). -!- In vitro the enzyme 2'-O-methylates cytidine(34) of tRNA(Ser1) and uridine(32) of tRNA(Gln2).

UniProtKB Entries (1)

P0AE01

TRMJ_ECOLI

Escherichia coli K-12

TRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ

PDB Structure

PDB	4XBO
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	tRNA recognition by a bacterial tRNA Xm32 modification enzyme from the SPOUT methyltransferase superfamily Liu, R.J., Long, T., Zhou, M., Zhou, X.L., Wang, E.D. Nucleic Acids Res.