CATH Domain 4wzmA02

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.30	2-Layer Sandwich
	3.30.70	Alpha-Beta Plaits
	3.30.70.270	Reverse transcriptase/Diguanylate cyclase domain

Domain Context

CATH Clusters

Superfamily	3.30.70.270
Functional Family

Enzyme Information

3.4.22.46	L-peptidase. based on mapping to UniProt P03311 Autocatalytically cleaves itself from the polyprotein of the foot- and-mouth disease virus by hydrolysis of a Lys-\|-Gly bond, but then cleaves host cell initiation factor eIF-4G at bonds -Gly-\|-Arg- and -Lys-\|-Arg-. -!- Best known from foot-and-mouth disease virus, but occurs in other aphthoviruses and cardioviruses. -!- Destruction of initiation factor eIF-4G has the effect of shutting off host-cell protein synthesis while allowing synthesis of viral proteins to continue. -!- The tertiary structure reveals a distant relationship to papain and, consistent with this, compound E-64 is inhibitory. -!- Belongs to peptidase family C28.
2.7.7.48	RNA-directed RNA polymerase. based on mapping to UniProt P03311 Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). -!- Catalyzes RNA-template-directed extension of the 3'-end of an RNA strand by one nucleotide at a time. -!- Can initiate a chain de novo. -!- See also EC 2.7.7.6.
3.4.22.28	Picornain 3C. based on mapping to UniProt P03311 Selective cleavage of Gln-\|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly. -!- From entero-, rhino-, aphto- and cardioviruses. -!- Larger than the homologous virus picornain 2A. -!- Belongs to peptidase family C3.
3.6.1.15	Nucleoside-triphosphate phosphatase. based on mapping to UniProt P03311 NTP + H(2)O = NDP + phosphate. -!- The enzyme is found in eukaryotes and thermophilic bacteria, but appears to be absent from mesophilic bacteria. -!- Also hydrolyzes nucleoside diphosphates, thiamine diphosphate and FAD. -!- The enzyme from the plant Pisum sativum (garden pea) is regulated by calmodulin.

UniProtKB Entries (1)

POLG_FMDVS

Foot-and-mouth disease virus (strain C1-Santa Pau)

Genome polyprotein

PDB Structure

PDB	4WZM
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Multifunctionality of a picornavirus polymerase domain: nuclear localization signal and nucleotide recognition. Ferrer-Orta, C., de la Higuera, I., Caridi, F., Sanchez-Aparicio, M.T., Moreno, E., Perales, C., Singh, K., Sarafianos, S.G., Sobrino, F., Domingo, E., Verdaguer, N. J.Virol.