CATH Domain 4piiA01

CATH Classification

Level	CATH Code	Description
	1	Mainly Alpha
	1.10	Orthogonal Bundle
	1.10.1670	Endonuclease Iii, domain 2
	1.10.1670.10	Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)

Domain Context

CATH Clusters

Superfamily	Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)
Functional Family

Enzyme Information

3.2.2.-

Hydrolyzing N-glycosyl compounds.

based on mapping to UniProt Q8U2D5

4.2.99.18

DNA-(apurinic or apyrimidinic site) lyase.

based on mapping to UniProt Q8U2D5

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

-!- 'Nicking' of the phosphodiester bond is due to a lyase-type reaction, not hydrolysis. -!- This group of enzymes was previously listed as endonucleases, under the number EC 3.1.25.2.

UniProtKB Entries (1)

Q8U2D5

AGOG_PYRFU

Pyrococcus furiosus DSM 3638

N-glycosylase/DNA lyase

PDB Structure

PDB	4PII
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Fast native-SAD phasing for routine macromolecular structure determination. Weinert, T., Olieric, V., Waltersperger, S., Panepucci, E., Chen, L., Zhang, H., Zhou, D., Rose, J., Ebihara, A., Kuramitsu, S., Li, D., Howe, N., Schnapp, G., Pautsch, A., Bargsten, K., Prota, A.E., Surana, P., Kottur, J., Nair, D.T., Basilico, F., Cecatiello, V., Pasqualato, S., Boland, A., Weichenrieder, O., Wang, B.C., Steinmetz, M.O., Caffrey, M., Wang, M. Nat.Methods