CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.40 | 3-Layer(aba) Sandwich |
|
3.40.50 | Rossmann fold |
|
3.40.50.620 | HUPs |
Domain Context
CATH Clusters
| Superfamily | HUPs |
| Functional Family | Probable tRNA sulfurtransferase |
Enzyme Information
| 2.8.1.4 |
tRNA uracil 4-sulfurtransferase.
based on mapping to UniProt Q9X220
ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster.
-!- The enzyme, found in bacteria and archaea, is activated by EC 2.8.1.7, which transfers a sulfur atom to an internal L-cysteine residue, forming a cysteine persulfide. -!- The activated enzyme then transfers the sulfur to a uridine in a tRNA chain in a reaction that requires ATP. -!- The enzyme from the bacterium Escherichia coli forms 4-thiouridine only at position 8 of tRNA. -!- The enzyme also participates in the biosynthesis of the thiazole moiety of thiamine, but different domains are involved in the two processes.
|
UniProtKB Entries (1)
| Q9X220 |
THII_THEMA
Thermotoga maritima MSB8
Probable tRNA sulfurtransferase
|
PDB Structure
| PDB | 4KR7 |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Crystal structure of a 4-thiouridine synthetase-RNA complex reveals specificity of tRNA U8 modification.
Nucleic Acids Res.
|
