CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.50 | 3-Layer(bba) Sandwich |
|
3.50.50 | FAD/NAD(P)-binding domain |
|
3.50.50.60 | FAD/NAD(P)-binding domain |
Domain Context
CATH Clusters
| Superfamily | FAD/NAD(P)-binding domain |
| Functional Family |
Enzyme Information
| 1.14.13.234 |
12-dehydrotetracycline 5-monooxygenase.
based on mapping to UniProt L8EUQ6
12-dehydrotetracycline + NADPH + O(2) = 5a,11a-dehydrooxytetracycline + NADP(+) + H(2)O.
-!- The enzyme, characterized from the bacterium Streptomyces rimosus, is bifunctional, catalyzing two successive monooxygenation reactions. -!- It starts by catalyzing the stereospecific hydroxylation of anhydrotetracycline at C(6) (EC 1.14.13.38). -!- If the released product is captured by EC 1.3.98.4 it is reduced to tetracycline. -!- However, if the released product is recaptured by OxyS, it performs an additional hydroxylation at C(5), producing 5a,11a- dehydrooxytetracycline, which, following the action of EC 1.3.98.4, becomes oxytetracycline.
|
| 1.14.13.38 |
Anhydrotetracycline 6-monooxygenase.
based on mapping to UniProt L8EUQ6
Anhydrotetracycline + NADPH + O(2) = 12-dehydrotetracycline + NADP(+) + H(2)O.
-!- The enzyme, characterized from the bacterium Streptomyces rimosus, participates in the biosynthesis of tetracycline antibiotics. -!- It can also catalyze EC 1.14.13.234.
|
UniProtKB Entries (1)
| L8EUQ6 |
OXYS_STRR1
Streptomyces rimosus subsp. rimosus ATCC 10970
12-dehydrotetracycline 5-monooxygenase/anhydrotetracycline 6-monooxygenase
|
PDB Structure
| PDB | 4K2X |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Uncovering the Enzymes that Catalyze the Final Steps in Oxytetracycline Biosynthesis.
J.Am.Chem.Soc.
|
