CATH Classification

Domain Context

CATH Clusters

Superfamily Cytochrome P450
Functional Family cholesterol 24-hydroxylase isoform X2

Enzyme Information

1.14.14.25
Cholesterol 24-hydroxylase.
based on mapping to UniProt Q9Y6A2
Cholesterol + [reduced NADPH--hemoprotein reductase] + O(2) = (24S)- cholest-5-ene-3-beta,24-diol + [oxidized NADPH--hemoprotein reductase] + H(2)O.
-!- The enzyme can also produce 25-hydroxycholesterol. -!- In addition, it can further hydroxylate the product to 24,25- dihydroxycholesterol and 24,27-dihydroxycholesterol. -!- This reaction is the first step in the enzymic degradation of cholesterol in the brain as hydroxycholesterol can pass the blood- brain barrier whereas cholesterol cannot. -!- The direct electron donor to the enzyme is EC 1.6.2.4. -!- Formerly EC 1.14.13.98.

UniProtKB Entries (1)

Q9Y6A2
CP46A_HUMAN
Homo sapiens
Cholesterol 24-hydroxylase

PDB Structure

PDB 4J14
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Antifungal Azoles: Structural Insights into Undesired Tight Binding to Cholesterol-Metabolizing CYP46A1.
Mast, N., Zheng, W., Stout, C.D., Pikuleva, I.A.
Mol.Pharmacol.