CATH Classification
Level | CATH Code | Description |
---|---|---|
1 | Mainly Alpha | |
1.10 | Orthogonal Bundle | |
1.10.10 | Arc Repressor Mutant, subunit A | |
1.10.10.2660 | Ubiquitin-activating enzyme E1, SCCH domain |
Domain Context
CATH Clusters
Superfamily | Ubiquitin-activating enzyme E1, SCCH domain |
Functional Family | Ubiquitin-activating enzyme E1 1 |
Enzyme Information
6.2.1.45 |
E1 ubiquitin-activating enzyme.
based on mapping to UniProt O94609
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine.
-!- Catalyzes the ATP-dependent activation of ubiquitin through the formation of a thioester bond between the C-terminal glycine of ubiquitin and the sulfhydryl side group of a cysteine residue in the E1 protein. -!- The two-step reaction consists of the ATP-dependent formation of an E1-ubiquitin adenylate intermediate in which the C-terminal glycine of ubiquitin is bound to AMP via an acyl-phosphate linkage, then followed by the conversion to an E1-ubiquitin thioester bond via the cysteine residue on E1 in the second step. -!- Formerly EC 6.3.2.19 and EC 6.3.2.21.
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UniProtKB Entries (1)
P46595 |
UBC4_SCHPO
Schizosaccharomyces pombe 972h-
Ubiquitin-conjugating enzyme E2 4
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PDB Structure
PDB | 4II2 |
External Links | |
Method | X-RAY DIFFRACTION |
Organism | |
Primary Citation |
Structure of a ubiquitin E1-E2 complex: insights to E1-E2 thioester transfer.
Mol.Cell
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