CATH Domain 4gmcB03

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.30	2-Layer Sandwich
	3.30.70	Alpha-Beta Plaits
	3.30.70.270	Reverse transcriptase/Diguanylate cyclase domain

Domain Context

CATH Clusters

Superfamily	3.30.70.270
Functional Family

Enzyme Information

3.4.21.98	Hepacivirin. based on mapping to UniProt O92972 Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'. -!- Encoded by the genome of the viruses of the hepatitis C group, and contributes to the maturation of the precursor polyproteins. -!- The enzyme is greatly activated by binding of the 54-residue NS4A 'cofactor' protein also derived from the viral polyprotein. -!- Belongs to peptidase family S29.
3.6.4.13	RNA helicase. based on mapping to UniProt O92972 ATP + H(2)O = ADP + phosphate. -!- RNA helicases utilize the energy from ATP hydrolysis to unwind RNA. -!- Some of them unwind RNA with a 3' to 5' polarity, other show 5' to 3' polarity. -!- Some helicases unwind DNA as well as RNA. -!- May be identical with EC 3.6.4.12 (DNA helicase).
3.6.1.15	Nucleoside-triphosphate phosphatase. based on mapping to UniProt O92972 NTP + H(2)O = NDP + phosphate. -!- The enzyme is found in eukaryotes and thermophilic bacteria, but appears to be absent from mesophilic bacteria. -!- Also hydrolyzes nucleoside diphosphates, thiamine diphosphate and FAD. -!- The enzyme from the plant Pisum sativum (garden pea) is regulated by calmodulin.
2.7.7.48	RNA-directed RNA polymerase. based on mapping to UniProt O92972 Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). -!- Catalyzes RNA-template-directed extension of the 3'-end of an RNA strand by one nucleotide at a time. -!- Can initiate a chain de novo. -!- See also EC 2.7.7.6.
3.4.22.-	Cysteine endopeptidases. based on mapping to UniProt O92972

UniProtKB Entries (1)

POLG_HCVJ4

Hepatitis C virus isolate HC-J4

Genome polyprotein

PDB Structure

PDB	4GMC
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Allosteric N-acetamide-indole-6-carboxylic acid thumb pocket 1 inhibitors of hepatitis C virus NS5B polymerase - Acylsulfonamides and acylsulfamides as carboxylic acid replacements Beaulieu, P.L., Coulombe, R., Gillard, J., Brochu, C., Duan, J., Garneau, M., Jolicoeur, E., Kuhn, P., Poupart, M.A., Rancourt, J., Stammers, T.A., Thavonekham, B., Kukolj, G. Can.J.Chem.