CATH Classification
Level | CATH Code | Description |
---|---|---|
![]() |
1 | Mainly Alpha |
![]() |
1.20 | Up-down Bundle |
![]() |
1.20.120 | Four Helix Bundle (Hemerythrin (Met), subunit A) |
![]() |
1.20.120.1790 |
Domain Context
CATH Clusters
Superfamily | 1.20.120.1790 |
Functional Family | roquin-1 isoform X1 |
Enzyme Information
2.3.2.27 |
RING-type E3 ubiquitin transferase.
based on mapping to UniProt Q5TC82
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine.
-!- RING E3 ubiquitin transferases serve as mediators bringing the ubiquitin-charged E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) and an acceptor protein together to enable the direct transfer of ubiquitin through the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon-amino group of an L-lysine residue of the acceptor protein. -!- Unlike EC 2.3.2.26 the RING-E3 domain does not form a catalytic thioester intermediate with ubiquitin. -!- Many members of the RING-type E3 ubiquitin transferase family are not able to bind a substrate directly, and form a complex with a cullin scaffold protein and a substrate recognition module (the complexes are named CRL for Cullin-RING-Ligase). -!- In these complexes, the RING-type E3 ubiquitin transferase provides an additional function, mediating the transfer of a NEDD8 protein from a dedicated E2 carrier to the cullin protein (see EC 2.3.2.32). -!- Cf. EC 2.3.2.31.
|
UniProtKB Entries (1)
Q5TC82 |
RC3H1_HUMAN
Homo sapiens
Roquin-1
|
PDB Structure
PDB | 3X1O |
External Links | |
Method | X-RAY DIFFRACTION |
Organism | |
Primary Citation |
Roquin binds microRNA-146a and Argonaute2 to regulate microRNA homeostasis
Nat Commun
|