CATH Domain 3w0qA02

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.90	Alpha-Beta Complex
	3.90.1200	Aminoglycoside 3'-phosphotransferase; Chain: A, domain 2
	3.90.1200.10	Aminoglycoside phosphotransferase (APH), C-terminal lobe

Domain Context

CATH Clusters

Superfamily	3.90.1200.10
Functional Family

Enzyme Information

2.7.1.163

Hygromycin B 4-O-kinase.

based on mapping to UniProt P00557

ATP + hygromycin B = ADP + 4-O-phosphohygromycin B.

-!- Phosphorylates the antibiotic hygromycin B. -!- Whereas the enzyme from Streptomyces hygroscopicus (EC 2.7.1.119) catalyzes the formation of 7''-O-phosphohygromycin B, this enzyme, found in Escherichia coli carrying a plasmid conferring resistance to hygromycin-B, forms 4-O-phosphohygromycin B. -!- Formerly EC 2.7.1.n6.

UniProtKB Entries (1)

P00557

KHYB_ECOLX

Escherichia coli

Hygromycin-B 4-O-kinase

PDB Structure

PDB	3W0Q
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Crystal structures of the ternary complex of APH(4)-Ia/Hph with hygromycin B and an ATP analog using a thermostable mutant. Iino, D., Takakura, Y., Fukano, K., Sasaki, Y., Hoshino, T., Ohsawa, K., Nakamura, A., Yajima, S. J.Struct.Biol.