CATH Domain 3vf4A03

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.90	Alpha-Beta Complex
	3.90.870	DHBP synthase
	3.90.870.20	Carbamoyltransferase, C-terminal domain

Domain Context

CATH Clusters

Superfamily	Carbamoyltransferase, C-terminal domain
Functional Family	Decarbamoylnovobiocin carbamoyltransferase

Enzyme Information

6.1.2.2

Nebramycin 5' synthase.

based on mapping to UniProt Q70IY1

(1) Tobramycin + carbamoyl phosphate + ATP + H(2)O = nebramycin 5' + AMP + diphosphate + phosphate. (2) Kanamycin A + carbamoyl phosphate + ATP + H(2)O = 6''-O- carbamoylkanamycin A + AMP + diphosphate + phosphate.

-!- The enzyme from the bacterium Streptoalloteichus tenebrarius catalyzes the activation of carbamoyl phosphate to O-carbamoyladenylate and the subsequent carbamoylation of kanamycin and tobramycin. -!- Formerly EC 2.1.3.13 and EC 2.1.3.14.

UniProtKB Entries (1)

Q70IY1

TOBZ_STRSD

Streptoalloteichus tenebrarius

Nebramycin 5' synthase

PDB Structure

PDB	3VF4
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	The O-Carbamoyltransferase TobZ Catalyzes an Ancient Enzymatic Reaction. Parthier, C., Gorlich, S., Jaenecke, F., Breithaupt, C., Brauer, U., Fandrich, U., Clausnitzer, D., Wehmeier, U.F., Bottcher, C., Scheel, D., Stubbs, M.T. Angew.Chem.Int.Ed.Engl.