CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.30 | 2-Layer Sandwich |
|
3.30.300 | GMP Synthetase; Chain A, domain 3 |
|
3.30.300.30 | ANL, C-terminal domain |
Domain Context
CATH Clusters
| Superfamily | 3.30.300.30 |
| Functional Family | Enterobactin synthase component E |
Enzyme Information
| 6.3.2.14 |
Enterobactin synthase.
based on mapping to UniProt P0ADI4
6 ATP + 3 2,3-dihydroxybenzoate + 3 L-serine = enterobactin + 6 AMP + 6 diphosphate.
-!- This enzyme complex catalyzes the conversion of three molecules each of 2,3-dihydroxybenzoate and L-serine to form the siderophore enterobactin. -!- In Escherichia coli the complex is formed by EntB (an aryl carrier protein that has to be activated by 4'-phosphopantetheine), EntD (a phosphopantetheinyl transferase that activates EntB), EntE (catalyzes the ATP-dependent condensation of 2,3-dihydroxybenzoate and holo-EntB to form the covalently arylated form of EntB), and EntF (a four domain protein that catalyzes the activation of L-serine by ATP, the condensation of the activated L-serine with the activated 2,3- dihydroxybenzoate, and the trimerization of three such moieties to a single enterobactin molecule).
|
| 3.3.2.1 |
Isochorismatase.
based on mapping to UniProt P0ADI4
Isochorismate + H(2)O = (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate.
|
| 2.5.1.- |
Transferring alkyl or aryl groups, other than methyl groups.
based on mapping to UniProt P10378
|
| 2.7.7.58 |
(2,3-dihydroxybenzoyl)adenylate synthase.
based on mapping to UniProt P10378
ATP + 2,3-dihydroxybenzoate = diphosphate + (2,3- dihydroxybenzoyl)adenylate.
|
| 6.3.2.14 |
Enterobactin synthase.
based on mapping to UniProt P10378
6 ATP + 3 2,3-dihydroxybenzoate + 3 L-serine = enterobactin + 6 AMP + 6 diphosphate.
-!- This enzyme complex catalyzes the conversion of three molecules each of 2,3-dihydroxybenzoate and L-serine to form the siderophore enterobactin. -!- In Escherichia coli the complex is formed by EntB (an aryl carrier protein that has to be activated by 4'-phosphopantetheine), EntD (a phosphopantetheinyl transferase that activates EntB), EntE (catalyzes the ATP-dependent condensation of 2,3-dihydroxybenzoate and holo-EntB to form the covalently arylated form of EntB), and EntF (a four domain protein that catalyzes the activation of L-serine by ATP, the condensation of the activated L-serine with the activated 2,3- dihydroxybenzoate, and the trimerization of three such moieties to a single enterobactin molecule).
|
UniProtKB Entries (1)
| P10378 |
ENTE_ECOLI
Escherichia coli K-12
Enterobactin synthase component E
|
PDB Structure
| PDB | 3RG2 |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Structural and Functional Investigation of the Intermolecular Interaction between NRPS Adenylation and Carrier Protein Domains.
Chem.Biol.
|
