CATH Classification

Domain Context

CATH Clusters

Superfamily 1.10.287.4290
Functional Family Peroxisomal multifunctional enzyme type 2

Enzyme Information

1.1.1.n12
(3R)-hydroxyacyl-CoA dehydrogenase.
based on mapping to UniProt Q9VXJ0
(R)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH.
4.2.1.119
Enoyl-CoA hydratase 2.
based on mapping to UniProt Q9VXJ0
(3R)-3-hydroxyacyl-CoA = (2E)-2-enoyl-CoA + H(2)O.
-!- This enzyme catalyzes a hydration step in peroxisomal beta-oxidation. -!- The human multifunctional enzyme type 2 (MFE-2) is a 79000 Da enzyme composed of three functional units: (3R)-hydroxyacyl-CoA dehydrogenase, 2-enoyl-CoA hydratase 2 and sterol carrier protein 2-like units. -!- The enzymes from Aeromonas caviae and Arabidopsis thaliana are monofunctional enzymes. -!- 2-enoyl-CoA hydratase 3 from Candida tropicalis is a part from multifunctional enzyme type 2.

UniProtKB Entries (1)

Q9VXJ0
DHB4_DROME
Drosophila melanogaster
Peroxisomal multifunctional enzyme type 2

PDB Structure

PDB 3OML
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Peroxisomal multifunctional enzyme type 2 from the fruitfly: dehydrogenase and hydratase act as separate entities, as revealed by structure and kinetics.
Haataja, T.J., Koski, M.K., Hiltunen, J.K., Glumoff, T.
Biochem.J.