CATH Classification

Domain Context

CATH Clusters

Superfamily endopeptidase domain like (from Nostoc punctiforme)
Functional Family Bifunctional glutathionylspermidine synthetase/amidase

Enzyme Information

3.5.1.78
Glutathionylspermidine amidase.
based on mapping to UniProt P0AES0
Glutathionylspermidine + H(2)O = glutathione + spermidine.
-!- Transforms glutathionylspermidine into glutathione and spermidine. -!- The enzyme from Escherichia coli is bifunctional and also catalyzes the reaction of EC 6.3.1.8, resulting in a net hydrolysis of ATP.
6.3.1.8
Glutathionylspermidine synthase.
based on mapping to UniProt P0AES0
Glutathione + spermidine + ATP = glutathionylspermidine + ADP + phosphate.
-!- Involved in the synthesis of trypanothione in trypanosomatids. -!- The enzyme from Escherichia coli is bifunctional and also catalyzes the EC 3.5.1.78 reaction, resulting in a net hydrolysis of ATP.

UniProtKB Entries (1)

P0AES0
GSP_ECOLI
Escherichia coli K-12
Bifunctional glutathionylspermidine synthetase/amidase

PDB Structure

PDB 3O98
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structure and mechanism of Escherichia coli glutathionylspermidine amidase belonging to the family of cysteine; histidine-dependent amidohydrolases/peptidases
Pai, C.-H., Wu, H.-J., Lin, C.-H., Wang, A.H.-J.
Protein Sci.