CATH Domain 3nw2A03

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.90	Alpha-Beta Complex
	3.90.1230	Bovine Endothelial Nitric Oxide Synthase Heme Domain; Chain: A,domain 3
	3.90.1230.10	Nitric Oxide Synthase; Chain A, domain 3

Domain Context

CATH Clusters

Superfamily	Nitric Oxide Synthase; Chain A, domain 3
Functional Family	Nitric oxide synthase, brain

Enzyme Information

1.14.13.39

Nitric-oxide synthase (NADPH).

based on mapping to UniProt P29477

2 L-arginine + 3 NADPH + 4 O(2) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H(2)O.

-!- The enzyme consists of linked oxygenase and reductase domains. -!- The eukaryotic enzyme binds FAD, FMN, heme (iron protoporphyrin IX) and tetrahydrobiopterin, and its two domains are linked via a regulatory calmodulin-binding domain. -!- Upon calcium-induced calmodulin binding, the reductase and oxygenase domains form a complex, allowing electrons to flow from NADPH via FAD and FMN to the active center. -!- The reductase domain of the enzyme from the bacterium Sorangium cellulosum utilizes a [2Fe-2S] cluster to transfer the electrons from NADPH to the active center. -!- Cf. EC 1.14.14.47.

UniProtKB Entries (1)

P29477

NOS2_MOUSE

Mus musculus

Nitric oxide synthase, inducible

PDB Structure

PDB	3NW2
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Novel nanomolar imidazo[4,5-b]pyridines as selective nitric oxide synthase (iNOS) inhibitors: SAR and structural insights Graedler, U., Fuchss, T., Ulrich, W.R., Boer, R., Strub, A., Hesslinger, C., Anezo, C., Diederichs, K., Zaliani, A. Bioorg.Med.Chem.Lett.