CATH Classification

Domain Context

CATH Clusters

Superfamily Cytochrome P450
Functional Family

Enzyme Information

1.14.14.154
Sterol 14-alpha-demethylase.
based on mapping to UniProt Q7Z1V1
A 14-alpha-methylsteroid + 3 [reduced NADPH--hemoprotein reductase] + 3 O(2) = a Delta(14)-steroid + formate + 3 [oxidized NADPH--hemoprotein reductase] + 4 H(2)O.
-!- This cytochrome P450 (heme-thiolate) enzyme acts on a range of steroids with a 14-alpha-methyl group, such as obtusifoliol and lanosterol. -!- The enzyme catalyzes a hydroxylation and a reduction of the 14-alpha- methyl group, followed by a second hydroxylation, resulting in the elimination of formate and formation of a 14(15) double bond. -!- Formerly EC 1.14.13.70.

UniProtKB Entries (1)

Q7Z1V1
CP51_TRYCC
Trypanosoma cruzi strain CL Brener
Sterol 14-alpha demethylase

PDB Structure

PDB 3KHM
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural Insights into Inhibition of Sterol 14{alpha}-Demethylase in the Human Pathogen Trypanosoma cruzi.
Lepesheva, G.I., Hargrove, T.Y., Anderson, S., Kleshchenko, Y., Furtak, V., Wawrzak, Z., Villalta, F., Waterman, M.R.
J.Biol.Chem.