CATH Classification

Domain Context

CATH Clusters

Superfamily 2.40.160.20
Functional Family Lipid A palmitoyltransferase PagP

Enzyme Information

2.3.1.251
Lipid IV(A) palmitoyltransferase.
based on mapping to UniProt P37001
(1) 1-palmitoyl-2-acyl-sn-glycero-3-phosphocholine + hexa-acyl lipid A = 2-acyl-sn-glycero-3-phosphocholine + hepta-acyl lipid A. (2) 1-palmitoyl-2-acyl-sn-glycero-3-phosphocholine + lipid II(A) = 2-acyl-sn-glycero-3-phosphocholine + lipid II(B). (3) 1-palmitoyl-2-acyl-sn-glycero-3-phosphocholine + lipid IV(A) = 2-acyl-sn-glycero-3-phosphocholine + lipid IV(B).
-!- Isolated from the bacteria Escherichia coli and Salmonella typhimurium. -!- The enzyme prefers phosphatidylcholine with a palmitoyl group at the sn-1 position and palmitoyl or stearoyl groups at the sn-2 position. -!- There is some activity with corresponding phosphatidylserines but only weak activity with other diacylphosphatidyl compounds. -!- The enzyme also acts on Kdo-(2->4)-Kdo-(2->6)-lipid IV(A).

UniProtKB Entries (1)

P37001
PAGP_ECOLI
Escherichia coli K-12
Lipid A palmitoyltransferase PagP

PDB Structure

PDB 3GP6
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
PagP crystallized from SDS/cosolvent reveals the route for phospholipid access to the hydrocarbon ruler.
Cuesta-Seijo, J.A., Neale, C., Khan, M.A., Moktar, J., Tran, C.D., Bishop, R.E., Pomes, R., Prive, G.G.
Structure