CATH Classification

Domain Context

CATH Clusters

Superfamily 3.90.70.80
Functional Family Ubiquitin thioesterase OTU1

Enzyme Information

3.4.19.12
Ubiquitinyl hydrolase 1.
based on mapping to UniProt P43558
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
-!- Links to polypeptides smaller than 60 residues are hydrolyzed more readily than those to larger polypeptides. -!- Isoforms exist with quantitatively different specificities among the best known being UCH-L1 and UCH-L3, major proteins of the brain of mammals. -!- Inhibited by ubiquitin aldehyde (in which Gly76 is replaced by aminoacetaldehyde). -!- Belongs to peptidase family C12.

UniProtKB Entries (2)

P0CG48
UBC_HUMAN
Homo sapiens
Polyubiquitin-C
P43558
OTU1_YEAST
Saccharomyces cerevisiae S288C
Ubiquitin thioesterase OTU1

PDB Structure

PDB 3C0R
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structural basis for ubiquitin recognition by the Otu1 ovarian tumor domain protein
Messick, T.E., Russell, N.S., Iwata, A.J., Sarachan, K.L., Shiekhattar, R., Shanks, J.R., Reyes-Turcu, F.E., Wilkinson, K.D., Marmorstein, R.
J.Biol.Chem.