CATH Domain 3budA03

CATH Classification

Level	CATH Code	Description
	2	Mainly Beta
	2.60	Sandwich
	2.60.40	Immunoglobulin-like
	2.60.40.1180	Golgi alpha-mannosidase II

Domain Context

CATH Clusters

Superfamily	Golgi alpha-mannosidase II
Functional Family	Alpha-mannosidase 2

Enzyme Information

3.2.1.114

Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase.

based on mapping to UniProt Q24451

Man(5)GlcNAc(3)-[protein] + 2 H(2)O = Man(3)GlcNAc(3)-[protein] + 2 alpha-D-mannopyranose.

-!- The enzyme, found in plants and animals, participates in the processing of N-glycans in the Golgi apparatus. -!- It removes two mannosyl residues, one linked by alpha-1,3 linkage, and the other linked by alpha-1,6 linkage, both of which are removed by the same catalytic site. -!- The enzyme is sensitive to swainsonine.

UniProtKB Entries (1)

Q24451

MAN2_DROME

Drosophila melanogaster

Alpha-mannosidase 2

PDB Structure

PDB	3BUD
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Probing the substrate specificity of Golgi alpha-mannosidase II by use of synthetic oligosaccharides and a catalytic nucleophile mutant. Zhong, W., Kuntz, D.A., Ember, B., Singh, H., Moremen, K.W., Rose, D.R., Boons, G.J. J.Am.Chem.Soc.