CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.40 | 3-Layer(aba) Sandwich |
|
3.40.50 | Rossmann fold |
|
3.40.50.300 | P-loop containing nucleotide triphosphate hydrolases |
Domain Context
CATH Clusters
| Superfamily | P-loop containing nucleotide triphosphate hydrolases |
| Functional Family | Sulfotransferase |
Enzyme Information
| 2.8.2.23 |
[Heparan sulfate]-glucosamine 3-sulfotransferase 1.
based on mapping to UniProt Q8IZT8
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate.
-!- This enzyme differs from EC 2.8.2.29 and EC 2.8.2.30 by being the most selective for a precursor of the antithrombin-binding site. -!- It has a minimal acceptor sequence of: -> GlcNAc6S->GlcA-> GlcN2S*+-6S->IdoA2S->GlcN2S->. -!- It can also modify other precursor sequences within heparan sulfate but this action does not create functional antithrombin-binding sites. -!- These precursors are variants of the consensus sequence: -> Glc(N2S>NAc)+-6S->GlcA->GlcN2S*+-6S->GlcA>IdoA+-2S-> Glc(N2S/NAc)+-6S->. -!- If the heparan sulfate substrate lacks 2-O-sulfation of GlcA residues, then enzyme specificity is expanded to modify selected glucosamine residues preceded by IdoA as well as GlcA.
|
UniProtKB Entries (1)
| Q8IZT8 |
HS3S5_HUMAN
Homo sapiens
Heparan sulfate glucosamine 3-O-sulfotransferase 5
|
PDB Structure
| PDB | 3BD9 |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | Escherichia |
| Primary Citation |
Engineering sulfotransferases to modify heparan sulfate.
Nat.Chem.Biol.
|
