CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.30 | 2-Layer Sandwich |
|
3.30.56 | Phenylalanyl-tRNA Synthetase; Chain B, domain 1 |
|
3.30.56.70 | N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain |
Domain Context
CATH Clusters
| Superfamily | N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain |
| Functional Family | tRNA (guanine(26)-N(2)/guanine(27)-N(2))-dimethyltransferase |
Enzyme Information
| 2.1.1.215 |
tRNA (guanine(26)-N(2)/guanine(27)-N(2))-dimethyltransferase.
based on mapping to UniProt O67010
4 S-adenosyl-L-methionine + guanine(26)/guanine(27) in tRNA = 4 S-adenosyl-L-homocysteine + N(2)-dimethylguanine(26)/N(2)- dimethylguanine(27) in tRNA.
-!- The enzyme from Aquifex aeolicus is similar to the TRM1 methyltransferases of archaea and eukarya (see EC 2.1.1.216). -!- However, it catalyzes the double methylation of guanines at both positions 26 and 27 of tRNA. -!- Formerly EC 2.1.1.32.
|
UniProtKB Entries (1)
| O67010 |
TRM1_AQUAE
Aquifex aeolicus VF5
TRNA (guanine(26)-N(2)/guanine(27)-N(2))-dimethyltransferase
|
PDB Structure
| PDB | 3AXT |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Substrate tRNA recognition mechanism of a multisite-specific tRNA methyltransferase, Aquifex aeolicus Trm1, based on the X-ray crystal structure
J.Biol.Chem.
|