CATH Domain 3a30A00

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.90	Alpha-Beta Complex
	3.90.1720	endopeptidase fold (from Nostoc punctiforme)
	3.90.1720.10	endopeptidase domain like (from Nostoc punctiforme)

Domain Context

CATH Clusters

Superfamily	endopeptidase domain like (from Nostoc punctiforme)
Functional Family	Bifunctional glutathionylspermidine synthetase/amidase

Enzyme Information

3.5.1.78	Glutathionylspermidine amidase. based on mapping to UniProt P0AES0 Glutathionylspermidine + H(2)O = glutathione + spermidine. -!- Transforms glutathionylspermidine into glutathione and spermidine. -!- The enzyme from Escherichia coli is bifunctional and also catalyzes the reaction of EC 6.3.1.8, resulting in a net hydrolysis of ATP.
6.3.1.8	Glutathionylspermidine synthase. based on mapping to UniProt P0AES0 Glutathione + spermidine + ATP = glutathionylspermidine + ADP + phosphate. -!- Involved in the synthesis of trypanothione in trypanosomatids. -!- The enzyme from Escherichia coli is bifunctional and also catalyzes the EC 3.5.1.78 reaction, resulting in a net hydrolysis of ATP.

UniProtKB Entries (1)

GSP_ECOLI

Escherichia coli K-12

Bifunctional glutathionylspermidine synthetase/amidase

PDB Structure

PDB	3A30
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Protein S-thiolation by Glutathionylspermidine (Gsp): the role of Escherichia coli Gsp synthetASE/amidase in redox regulation Chiang, B.-Y., Chen, T.-C., Pai, C.-H., Chou, C.-C., Chen, H.-H., Ko, T.-P., Hsu, W.-H., Chang, C.-Y., Wu, W.-F., Wang, A.H.-J., Lin, C.-H. J.Biol.Chem.