CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.630.30
Functional Family tRNA(Met) cytidine acetyltransferase TmcA

Enzyme Information

2.3.1.193
tRNA(Met) cytidine acetyltransferase.
based on mapping to UniProt P76562
(Elongator tRNA(Met))-cytidine(34) + ATP + acetyl-CoA + H(2)O = (elongator tRNA(Met))-N(4)-acetylcytidine(34) + ADP + phosphate + CoA.
-!- The enzyme acetylates the wobble base C34 of the CAU anticodon of elongation-specific tRNA(Met). -!- Escherichia coli TmcA strictly discriminates elongator tRNA(Met) from tRNA(Ile), which is structurally similar and has the same anticodon loop, mainly by recognizing the C27-G43 pair in the anticodon stem. -!- The enzyme can use GTP in place of ATP for formation of N(4)- acetylcytidine.

UniProtKB Entries (1)

P76562
TMCA_ECOLI
Escherichia coli K-12
TRNA(Met) cytidine acetyltransferase TmcA

PDB Structure

PDB 2ZPA
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
RNA helicase module in an acetyltransferase that modifies a specific tRNA anticodon
Chimnaronk, S., Suzuki, T., Manita, T., Ikeuchi, Y., Yao, M., Suzuki, T., Tanaka, I.
Embo J.