CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.90 | Alpha-Beta Complex |
|
3.90.730 | Ribonuclease Rh; Chain A |
|
3.90.730.10 | Ribonuclease T2-like |
Domain Context
CATH Clusters
| Superfamily | Ribonuclease T2-like |
| Functional Family | Ribonuclease I |
Enzyme Information
| 4.6.1.21 |
Enterobacter ribonuclease.
based on mapping to UniProt P21338
RNA containing adenosine-cytidine + H(2)O = an (RNA fragment)- 3'-cytidine-3'-phosphate + a 5'-a hydroxy-adenosine -3'-(RNA fragment).
-!- Preference for cleavage at Cp-A bonds. -!- Homopolymers of A, U or G are not hydrolyzed. -!- CpG bonds are hydrolyzed less well and there is no detectable hydrolysis between two purines or two pyrimidines. -!- The enzyme catalyzes a two-stage endonucleolytic cleavage. -!- The first reaction produces 5'-hydroxy-phosphooligonucletides and 3'-phosphooligonucleotides ending a with 2',3'-cyclic phosphodiester, which are released from the enzyme. -!- The enzyme then hydrolyzes these cyclic compounds in a second reaction that takes place only when all the susceptible 3',5'-phosphodiester bonds have been cyclised. -!- The second reaction is a reversal of the first reaction using the hydroxyl group of water instead of the 5'-hydroxyl group of ribose. -!- The overall process is that of a phosphorus-oxygen lyase followed by hydrolysis to form the 3'-nucleotides. -!- Formerly EC 3.1.27.6.
|
UniProtKB Entries (1)
| P21338 |
RNI_ECOLI
Escherichia coli K-12
Ribonuclease I
|
PDB Structure
| PDB | 2Z70 |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Nonspecific base recognition mediated by water bridges and hydrophobic stacking in ribonuclease I from Escherichia coli
Protein Sci.
|
