CATH Classification

Domain Context

CATH Clusters

Superfamily Hect, E3 ligase catalytic domain
Functional Family E3 ubiquitin-protein ligase NEDD4-like

Enzyme Information

2.3.2.26
HECT-type E3 ubiquitin transferase.
based on mapping to UniProt Q9HAU4
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine.
-!- In the first step the enzyme transfers ubiquitin from the E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) to a cysteine residue in its HECT domain (which is located in the C-terminal region), forming a thioester bond. -!- In a subsequent step the enzyme transfers the ubiquitin to an acceptor protein, resulting in the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon- amino group of an L-lysine residue of the acceptor protein. -!- Cf. EC 2.3.2.27 and EC 2.3.2.31.

UniProtKB Entries (1)

Q9HAU4
SMUF2_HUMAN
Homo sapiens
E3 ubiquitin-protein ligase SMURF2

PDB Structure

PDB 1ZVD
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Regulation of Smurf2 Ubiquitin Ligase Activity by Anchoring the E2 to the HECT Domain.
Ogunjimi, A.A., Briant, D.J., Pece-Barbara, N., Le Roy, C., Di Guglielmo, G.M., Kavsak, P., Rasmussen, R.K., Seet, B.T., Sicheri, F., Wrana, J.L.
Mol.Cell