CATH Domain 1ylaA02

CATH Classification

Level	CATH Code	Description
	1	Mainly Alpha
	1.10	Orthogonal Bundle
	1.10.8	Helicase, Ruva Protein; domain 3
	1.10.8.10	DNA helicase RuvA subunit, C-terminal domain

Domain Context

CATH Clusters

Superfamily	DNA helicase RuvA subunit, C-terminal domain
Functional Family	Ubiquitin conjugating enzyme E2 K

Enzyme Information

2.3.2.23

E2 ubiquitin-conjugating enzyme.

based on mapping to UniProt P61086

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine.

-!- The E2 ubiquitin-conjugating enzyme acquires the activated ubquitin from the E1 ubiquitin-activating enzyme (EC 6.2.1.45) and binds it via a transthioesterification reaction to itself. -!- In the human enzyme the catalytic center is located at Cys-87 where ubiquitin is bound via its C-terminal glycine in a thioester linkage. -!- Formerly EC 6.3.2.19.

UniProtKB Entries (1)

P61086

UBE2K_HUMAN

Homo sapiens

Ubiquitin-conjugating enzyme E2 K

PDB Structure

PDB	1YLA
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Structural basis of E2-25K/UBB+1 interaction leading to proteasome inhibition and neurotoxicity Ko, S., Kang, G.B., Song, S.M., Lee, J.-G., Shin, D.Y., Yun, J.-H., Sheng, Y., Cheong, C., Jeon, Y.H., Jung, Y.-K., Arrowsmith, C.H., Avvakumov, G.V., Dhe-Paganon, S., Yoo, Y.J., Eom, S.H., Lee, W. J.Biol.Chem.