CATH Classification
Level | CATH Code | Description |
---|---|---|
3 | Alpha Beta | |
3.40 | 3-Layer(aba) Sandwich | |
3.40.50 | Rossmann fold | |
3.40.50.80 | Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module |
Domain Context
CATH Clusters
Superfamily | Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module |
Functional Family | Nitric oxide synthase |
Enzyme Information
1.14.13.39 |
Nitric-oxide synthase (NADPH).
based on mapping to UniProt P29476
2 L-arginine + 3 NADPH + 4 O(2) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H(2)O.
-!- The enzyme consists of linked oxygenase and reductase domains. -!- The eukaryotic enzyme binds FAD, FMN, heme (iron protoporphyrin IX) and tetrahydrobiopterin, and its two domains are linked via a regulatory calmodulin-binding domain. -!- Upon calcium-induced calmodulin binding, the reductase and oxygenase domains form a complex, allowing electrons to flow from NADPH via FAD and FMN to the active center. -!- The reductase domain of the enzyme from the bacterium Sorangium cellulosum utilizes a [2Fe-2S] cluster to transfer the electrons from NADPH to the active center. -!- Cf. EC 1.14.14.47.
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UniProtKB Entries (1)
P29476 |
NOS1_RAT
Rattus norvegicus
Nitric oxide synthase, brain
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PDB Structure
PDB | 1TLL |
External Links | |
Method | X-RAY DIFFRACTION |
Organism | Pichia |
Primary Citation |
Structural basis for isozyme-specific regulation of electron transfer in nitric-oxide synthase
J.Biol.Chem.
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