CATH Classification
Level | CATH Code | Description |
---|---|---|
3 | Alpha Beta | |
3.90 | Alpha-Beta Complex | |
3.90.226 | 2-enoyl-CoA Hydratase; Chain A, domain 1 | |
3.90.226.10 | 2-enoyl-CoA Hydratase; Chain A, domain 1 |
Domain Context
CATH Clusters
Superfamily | 2-enoyl-CoA Hydratase; Chain A, domain 1 |
Functional Family | 6-oxocamphor hydrolase |
Enzyme Information
3.7.1.18 |
6-oxocamphor hydrolase.
based on mapping to UniProt Q93TU6
Bornane-2,6-dione + H(2)O = ((1S)-4-hydroxy-2,2,3-trimethylcyclopent-3- enyl)acetate.
-!- Isolated from Rhodococcus sp. -!- The bornane ring system is cleaved by a retro-Claisen reaction to give the enol of alpha-campholonate. -!- When separate from the enzyme the enol is tautomerised to the keto form as a 6:1 mixture of ((1S,3R)-2,2,3-trimethyl-4- oxocyclopentyl)acetate and ((1S,3S)-2,2,3-trimethyl-4- oxocyclopentyl)acetate.
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UniProtKB Entries (1)
Q93TU6 |
CAMK_RHOSO
Rhodococcus sp.
6-oxocamphor hydrolase
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PDB Structure
PDB | 1SZO |
External Links | |
Method | X-RAY DIFFRACTION |
Organism | Escherichia |
Primary Citation |
Structure of 6-oxo camphor hydrolase H122A mutant bound to its natural product, (2S,4S)-alpha-campholinic acid: mutant structure suggests an atypical mode of transition state binding for a crotonase homolog.
J.Biol.Chem.
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