CATH Classification

Domain Context

CATH Clusters

Superfamily 2-enoyl-CoA Hydratase; Chain A, domain 1
Functional Family 6-oxocamphor hydrolase

Enzyme Information

3.7.1.18
6-oxocamphor hydrolase.
based on mapping to UniProt Q93TU6
Bornane-2,6-dione + H(2)O = ((1S)-4-hydroxy-2,2,3-trimethylcyclopent-3- enyl)acetate.
-!- Isolated from Rhodococcus sp. -!- The bornane ring system is cleaved by a retro-Claisen reaction to give the enol of alpha-campholonate. -!- When separate from the enzyme the enol is tautomerised to the keto form as a 6:1 mixture of ((1S,3R)-2,2,3-trimethyl-4- oxocyclopentyl)acetate and ((1S,3S)-2,2,3-trimethyl-4- oxocyclopentyl)acetate.

UniProtKB Entries (1)

Q93TU6
CAMK_RHOSO
Rhodococcus sp.
6-oxocamphor hydrolase

PDB Structure

PDB 1SZO
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structure of 6-oxo camphor hydrolase H122A mutant bound to its natural product, (2S,4S)-alpha-campholinic acid: mutant structure suggests an atypical mode of transition state binding for a crotonase homolog.
Leonard, P.M., Grogan, G.
J.Biol.Chem.