CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.30 | 2-Layer Sandwich |
|
3.30.70 | Alpha-Beta Plaits |
|
3.30.70.270 | Reverse transcriptase/Diguanylate cyclase domain |
Domain Context
CATH Clusters
| Superfamily | 3.30.70.270 |
| Functional Family | Genome polyprotein |
Enzyme Information
| 2.7.7.48 |
RNA-directed RNA polymerase.
based on mapping to UniProt P19711
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
-!- Catalyzes RNA-template-directed extension of the 3'-end of an RNA strand by one nucleotide at a time. -!- Can initiate a chain de novo. -!- See also EC 2.7.7.6.
|
| 3.4.21.113 |
Pestivirus NS3 polyprotein peptidase.
based on mapping to UniProt P19711
Leu is conserved at position P1 for all four cleavage sites. Alanine is found at position P1' of the NS4A-NS4B cleavage site, whereas serine is found at position P1' of the NS3-NS4A, NS4B-NS5A and NS5A-NS5B cleavage sites.
-!- The polyprotein of noncytopathogenic pestiviruses is cleaved co- and post-translationally into at least 11 proteins (N(pro), C, E(rns), E1, E2, p7, NS2-3, NS4A, NS4B, NS5A, and NS5B). -!- The genomes of cytopathogenic pestivirus strains express at least one additional protein, called NS3 (p80). -!- This enzyme, which resides in the N-terminal region of NS3 (nonstructural protein 3), is essential for generation of its own C-terminus and for processing of the downstream cleavage sites, leading to the release of the pestivirus nonstructural proteins NS4A, NS4B, NS5A and NS5B. -!- Belongs to peptidase family S31.
|
| 3.6.4.13 |
RNA helicase.
based on mapping to UniProt P19711
ATP + H(2)O = ADP + phosphate.
-!- RNA helicases utilize the energy from ATP hydrolysis to unwind RNA. -!- Some of them unwind RNA with a 3' to 5' polarity, other show 5' to 3' polarity. -!- Some helicases unwind DNA as well as RNA. -!- May be identical with EC 3.6.4.12 (DNA helicase).
|
| 3.6.1.15 |
Nucleoside-triphosphate phosphatase.
based on mapping to UniProt P19711
NTP + H(2)O = NDP + phosphate.
-!- The enzyme is found in eukaryotes and thermophilic bacteria, but appears to be absent from mesophilic bacteria. -!- Also hydrolyzes nucleoside diphosphates, thiamine diphosphate and FAD. -!- The enzyme from the plant Pisum sativum (garden pea) is regulated by calmodulin.
|
| 3.4.22.- |
Cysteine endopeptidases.
based on mapping to UniProt P19711
|
UniProtKB Entries (1)
| P19711 |
POLG_BVDVN
Bovine viral diarrhea virus 1-NADL
Genome polyprotein
|
PDB Structure
| PDB | 1S4F |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | Escherichia |
| Primary Citation |
The structure of the RNA-dependent RNA polymerase from bovine viral diarrhea virus establishes the role of GTP in de novo initiation.
Proc.Natl.Acad.Sci.Usa
|
