CATH Domain 1erzB00

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.60	4-Layer Sandwich
	3.60.110	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase
	3.60.110.10	Carbon-nitrogen hydrolase

Domain Context

CATH Clusters

Superfamily	Carbon-nitrogen hydrolase
Functional Family	N-carbamoyl-D-amino acid hydrolase

Enzyme Information

3.5.1.77

N-carbamoyl-D-amino-acid hydrolase.

based on mapping to UniProt P60327

N-carbamoyl-D-amino acid + H(2)O = D-amino acid + NH(3) + CO(2).

-!- This enzyme, along with EC 3.5.1.87, EC 5.1.99.5 and EC 3.5.2.2, forms part of the reaction cascade known as the 'hydantoinase process', which allows the total conversion of D,L-5-monosubstituted hydantoins into optically pure D- or L-amino acids. -!- Has strict stereospecificity for N-carbamoyl-D-amino acids and does not act upon the corresponding L-amino acids or N-formyl amino acids, N-carbamoyl-sarcosine, -citrulline, -allantoin and -ureidopropanoate, which are substrates for other amidohydrolases.

UniProtKB Entries (1)

P60327

DCAS_AGRSK

Agrobacterium sp. KNK712

N-carbamoyl-D-amino acid hydrolase

PDB Structure

PDB	1ERZ
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism	Escherichia
Primary Citation	Crystal structure of N-carbamyl-D-amino acid amidohydrolase with a novel catalytic framework common to amidohydrolases. Nakai, T., Hasegawa, T., Yamashita, E., Yamamoto, M., Kumasaka, T., Ueki, T., Nanba, H., Ikenaka, Y., Takahashi, S., Sato, M., Tsukihara, T. Structure Fold.Des.