CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.20 | Alpha-Beta Barrel |
|
3.20.20 | TIM Barrel |
|
3.20.20.70 | Aldolase class I |
Domain Context
CATH Clusters
| Superfamily | Aldolase class I |
| Functional Family | Glutamate synthase, large subunit |
Enzyme Information
| 1.4.1.13 |
Glutamate synthase (NADPH).
based on mapping to UniProt Q05755
2 L-glutamate + NADP(+) = L-glutamine + 2-oxoglutarate + NADPH.
-!- The reaction takes place in the opposite direction. -!- The protein is composed of two subunits, alpha and beta. -!- The alpha subunit is composed of two domains, one hydrolyzing L-glutamine to NH(3) and L-glutamate (cf. EC 3.5.1.2), the other combining the produced NH(3) with 2-oxoglutarate to produce a second molecule of L-glutamate (cf. EC 1.4.1.4). -!- The beta subunit transfers electrons to the cosubstrate. -!- The NH(3) is channeled through a 31 A channel in the active protein. -!- In the absence of the beta subunit, coupling between the two domains of the alpha subunit is compromised and some ammonium can be produced. -!- In the intact alpha-beta complex, ammonia production only takes place as part of the overall reaction. -!- Formerly EC 2.6.1.53.
|
UniProtKB Entries (1)
| Q05755 |
GLTB_AZOBR
Azospirillum brasilense
Glutamate synthase [NADPH] large chain
|
PDB Structure
| PDB | 1EA0 |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Cross-Talk and Ammonia Channeling between Active Centers in the Unexpected Domain Arrangement of Glutamate Synthase
Structure
|