CATH Classification
Level | CATH Code | Description |
---|---|---|
1 | Mainly Alpha | |
1.10 | Orthogonal Bundle | |
1.10.45 | Vanillyl-alcohol Oxidase; Chain A, domain 4 | |
1.10.45.10 | Vanillyl-alcohol Oxidase; Chain A, domain 4 |
Domain Context
CATH Clusters
Superfamily | Vanillyl-alcohol Oxidase; Chain A, domain 4 |
Functional Family |
Enzyme Information
1.17.9.1 |
4-methylphenol dehydrogenase (hydroxylating).
based on mapping to UniProt P09788
4-methylphenol + 4 oxidized azurin + H(2)O = 4-hydroxybenzaldehyde + 4 reduced azurin + 4 H(+).
-!- This bacterial enzyme contains a flavin (FAD) subunit and a cytochrome c subunit. -!- The flavin subunit abstracts two hydrogen atoms from the substrate, forming a quinone methide intermediate, then hydrates the latter at the benzylic carbon with a hydroxyl group derived from water. -!- The protons are lost to the bulk solvent, while the electrons are passed to the heme on the cytochrome subunit, and from there to azurin, a small copper-binding protein that is co-localized with the enzyme in the periplasm. -!- The first hydroxylation forms 4-hydroxybenzyl alcohol; a second hydroxylation converts this into 4-hydroxybenzaldehyde. -!- Formerly EC 1.17.99.1.
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UniProtKB Entries (1)
P09787 |
CY4C_PSEPU
Pseudomonas putida
4-cresol dehydrogenase [hydroxylating] cytochrome c subunit
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PDB Structure
PDB | 1DII |
External Links | |
Method | X-RAY DIFFRACTION |
Organism | |
Primary Citation |
Structures of the flavocytochrome p-cresol methylhydroxylase and its enzyme-substrate complex: gated substrate entry and proton relays support the proposed catalytic mechanism.
J.Mol.Biol.
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