CATH Classification

Domain Context

CATH Clusters

Superfamily Vaccinia Virus protein VP39
Functional Family

Enzyme Information

2.1.1.57
Methyltransferase cap1.
based on mapping to UniProt P07617
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)- (purine-ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)- methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)- [mRNA].
-!- This enzyme catalyzes the methylation of the ribose on the first transcribed nucleotide of mRNA or snRNA molecules, which may be either guanosine or adenosine. -!- This methylation event is known as cap1, and occurs in all mRNAs and snRNAs of higher eukaryotes, including insects, vertebrates and their viruses. -!- The human enzyme can also methylate mRNA molecules that lack methylation on the capping 5'-triphosphoguanosine. -!- Formerly EC 2.1.1.58.

UniProtKB Entries (1)

P07617
MCE_VACCW
Vaccinia virus WR
Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase

PDB Structure

PDB 1BKY
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
mRNA cap recognition: dominant role of enhanced stacking interactions between methylated bases and protein aromatic side chains.
Hu, G., Gershon, P.D., Hodel, A.E., Quiocho, F.A.
Proc.Natl.Acad.Sci.USA