CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.20 | Alpha-Beta Barrel |
|
3.20.20 | TIM Barrel |
|
3.20.20.80 | Glycosidases |
Domain Context
CATH Clusters
| Superfamily | Glycosidases |
| Functional Family |
Enzyme Information
| 2.4.1.248 |
Cycloisomaltooligosaccharide glucanotransferase.
based on mapping to UniProt P94286
Cyclizes part of a (1->6)-alpha-D-glucan chain by formation of a (1->6)- alpha-D-glucosidic bond.
-!- Specific for (1->6)-alpha-D-glucans (dextrans) and, unlike cyclomaltodextrin glucanotransferase (EC 2.4.1.19), without activity toward (1->4)-alpha-D-glucans, such as amylose. -!- It also has no activity on oligosaccharides, such as amylopectin and pullulan, containing (1->6)-alpha-D-glucosidic linkages at branch points. -!- The enzyme from Bacillus circulans T-3040 has been shown to form cycloisomalto-oligosaccharides of three sizes (7, 8 and 9 glucose units). -!- It will also catalyze the disproportionation of two isomalto- oligosaccharides molecules to yield a series of isomalto- oligosachharides and the addition of D-glucose to cycloisomalto- oligosaccharides with ring opening to form isomalto-oligosaccharides.
|
UniProtKB Entries (1)
| P94286 |
CTA1_BACCI
Bacillus circulans
Cycloisomaltooligosaccharide glucanotransferase
|
PDB Structure
| PDB | 3WNM |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Structural elucidation of the cyclization mechanism of alpha-1,6-glucan by Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase.
J.Biol.Chem.
|
