CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
2 | Mainly Beta |
|
2.102 | 3-layer Sandwich |
|
2.102.10 | Rieske Iron-sulfur Protein |
|
2.102.10.10 | Rieske [2Fe-2S] iron-sulphur domain |
Domain Context
CATH Clusters
| Superfamily | Rieske [2Fe-2S] iron-sulphur domain |
| Functional Family | 3-ketosteroid-9-alpha-hydroxylase oxygenase subunit |
Enzyme Information
| 1.14.15.30 |
3-ketosteroid 9-alpha-monooxygenase.
based on mapping to UniProt P71875
Androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) + O(2) = 9-alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O.
-!- The enzyme is involved in the cholesterol degradation pathway of several bacterial pathogens, such as Mycobacterium tuberculosis. -!- It forms a two-component system with a ferredoxin reductase (KshB). -!- The enzyme contains a Rieske-type iron-sulfur center and non-heme iron. -!- The product of the enzyme is unstable, and spontaneously converts to 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione. -!- Formerly EC 1.14.13.142.
|
UniProtKB Entries (1)
| P71875 |
KSHA_MYCTU
Mycobacterium tuberculosis H37Rv
3-ketosteroid-9-alpha-monooxygenase, oxygenase component
|
PDB Structure
| PDB | 4QCK |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Substrate specificities and conformational flexibility of 3-ketosteroid 9 alpha-hydroxylases.
J.Biol.Chem.
|
