CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.10 | Roll |
|
3.10.20 | Ubiquitin-like (UB roll) |
|
3.10.20.90 | Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1 |
Domain Context
CATH Clusters
| Superfamily | Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1 |
| Functional Family | E3 ubiquitin-protein ligase parkin |
Enzyme Information
| 2.3.2.31 |
RBR-type E3 ubiquitin transferase.
based on mapping to UniProt O60260
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.
-!- RBR-type E3 ubiquitin transferases have two RING fingers separated by an internal motif (IBR, for In Between RING). -!- The enzyme interacts with the CRL (Cullin-RING ubiquitin Ligase) complexes formed by certain RING-type E3 ubiquitin transferase (see EC 2.3.2.27), which include a neddylated cullin scaffold protein and a substrate recognition module. -!- The RING1 domain binds an EC 2.3.2.23, and transfers the ubiquitin that is bound to it to an internal cysteine residue in the RING2 domain, followed by the transfer of the ubiquitin from RING2 to the substrate. -!- Once the substrate has been ubiquitinated by the RBR-type ligase, it can be ubiqutylated further using ubiquitin carried directly on E2 enzymes, in a reaction catalyzed by EC 2.3.2.27. -!- Activity of the RBR-type enzyme is dependent on neddylation of the cullin protein in the CRL complex. -!- Cf. EC 2.3.2.26, EC 2.3.2.27, and EC 2.3.2.32.
|
UniProtKB Entries (1)
| O60260 |
PRKN_HUMAN
Homo sapiens
E3 ubiquitin-protein ligase parkin
|
PDB Structure
| PDB | 5TR5 |
| External Links | |
| Method | SOLUTION NMR |
| Organism | |
| Primary Citation |
Structure of phosphorylated UBL domain and insights into PINK1-orchestrated parkin activation.
Proc. Natl. Acad. Sci. U.S.A.
|
